Southeast Asian J Trop Med Public Health 2002 Jun;33(2):246-54
Knowledge and behavior relating to malaria in malaria endemic villages of
Khammouane Province, Lao PDR.
Uza M, Phommpida S, Toma T, Takakura M, Manivong K, Bounyadeth S, Kobayashi J,
Koja Y, Ozasa Y, Miyagi I.
Department of Community Health Nursing, School of Health Sciences, University of
the Ryukyus, Nishihara, Okinawa, Japan. Email: [email protected]
In order to provide basic data for evaluation of malaria control measures, a
study on the knowledge and behavior of people regarding prevention of malaria
was carried out in 8 malaria endemic villages in Khammouane Province of Lao PDR
from 1999 to 2000. The total valid questionnaire respondents were 932, with a
mean age of 32.3 +/- 14.9. 43.7% of the respondents were illiterate. About 44%
of the respondents suffered from malaria in the past. About 55% of the
illiterate group slept in mosquito nets, compared to 75.4% for the educated
group. About 29% of the illiterate respondents had knowledge of malaria
transmission by mosquito bites, compared to 48.8% for the educated groups. Out
of 167 non-impregnated mosquito nets examined in two villages, 13 were in bad
condition having holes or leaks and 39 female mosquitos including Anopheles spp
were collected in these nets by early morning catches. Knowledge of malaria and
behavior in relation to the prevention of malaria were significantly related to
educational level. Health education as well as general education must be taken
into account for communities in malaria endemic areas to become more involved in
malaria control strategies.
Clinical and laboratory predictors of imported malaria in an
outpatient setting: An aid to medical decision making in returning
travelers with fever
D'Acremont V; Landry P; Mueller I; Pecoud A; Genton B
Univ Lausanne,Med Outpatient Clin, Travel Clin,Rue Cesar Roux 19/CH-1005
Lausanne//Switzerland/ (REPRINT); Univ Lausanne,Med Outpatient Clin,
Travel Clin,CH-1005 Lausanne//Switzerland/; Swiss Trop Inst,CH-4002
No evidence-based information exists to guide clinicians for
giving presumptive treatment to returning travelers when malaria is
strongly suspected on clinical grounds but laboratory confirmation is
not immediately available or is negative. A prospective study was
conducted in travelers or migrants who sought care for fever to
identify clinical and laboratory predictors of Plasmodium parasitemia.
A total of 336 questionnaires were collected (97 malaria case patients
and 239 controls). Multivariate regression analysis showed inadequate
prophylaxis, sweating, no abdominal pain, temperature greater than or
equal to 38degreesC, poor general health, enlarged spleen, leucocytes
less than or equal to 10 x 10(3)/L, platelets < 150 x 10(3)/L,
hemoglobin < 12 g/dL, and eosinophils less than or equal to 5% to be
associated with parasitemia. Enlarged spleen had the highest positive
likelihood ratio for a diagnosis of malaria (13.6), followed by
thrombopenia (11.0). Posttest probabilities for malaria were 85% with
enlarged spleen and 82% with thrombopenia. A rapid assessment can thus
help to decide whether a presumptive treatment should be given or not,
especially when the results of the parasitological examination are not
immediately available or are uncertain.
Int Q Community Health Educ 1999;18(4):391-413
Cognitive and behavioral factors in community-based malaria control in Malawi.
Glik DC, Rubardt M, Nwanyanwu O, Jere S, Chikoko A, Zhang W.
Monitoring of preventive health behaviors and their determinants at the level of
households is an important component in field research for malaria control
programs. Household members in 12 rural villages in south central Malawi
participated in a study to show change over time in perceptions and behaviors in
an area undergoing multiple malaria control interventions. Focus group
interviews were used initially to elicit the major concepts villagers used to
define and respond to the threat of malaria. A longitudinal household survey
(T1: n = 2460; T2: n = 2149) measured biomedical or traditional knowledge about
malaria causation and control, attitudes toward mosquitoes, barriers to taking
preventive actions, and preventive behaviors defined as sanitation, traditional,
or commercial methods to control mosquitoes. While adherence to traditional
beliefs and behaviors was found among some, the majority of respondents also had
adopted many biomedical beliefs and attitudes about malaria causation. Changes
reflected both national and local initiatives to improve health status and
control malaria. Behaviors changed over time and were associated with a village
level intervention and with structural, situational, and cognitive attributes of
Am J Clin Nutr 2002 Oct;76(4):805-812
Effect of zinc on the treatment of Plasmodium falciparum malaria in children:
a randomized controlled trial.
BACKGROUND: Zinc supplementation in young children has been associated with
reductions in the incidence and severity of diarrheal diseases, acute
respiratory infections, and malaria. OBJECTIVE: The objective was to evaluate
the potential role of zinc as an adjunct in the treatment of acute,
uncomplicated falciparum malaria; a multicenter, double-blind, randomized
placebo-controlled clinical trial was undertaken. DESIGN: Children (n = 1087)
aged 6 mo to 5 y were enrolled at sites in Ecuador, Ghana, Tanzania, Uganda, and
Zambia. Children with fever and >/=2000 asexual forms of Plasmodium falciparum/
micro L in a thick blood smear received chloroquine and were randomly assigned
to receive zinc (20 mg/d for infants, 40 mg/d for older children) or placebo for
4 d. RESULTS: There was no effect of zinc on the median time to reduction of
fever (zinc group: 24.2 h; placebo group: 24.0 h; P = 0.37), a >/=75% reduction
in parasitemia from baseline in the first 72 h in 73.4% of the zinc group and in
77.6% of the placebo group (P = 0.11), and no significant change in hemoglobin
concentration during the 3-d period of hospitalization and the 4 wk of
follow-up. Mean plasma zinc concentrations were low in all children at baseline
(zinc group: 8.54 +/- 3.93 micro mol/L; placebo group: 8.34 +/- 3.25 micro
mol/L), but children who received zinc supplementation had higher plasma zinc
concentrations at 72 h than did those who received placebo (10.95 +/- 3.63
compared with 10.16 +/- 3.25 micro mol/L, P < 0.001). CONCLUSION: Zinc does not
appear to provide a beneficial effect in the treatment of acute, uncomplicated
falciparum malaria in preschool children.
J Med Invest 2002 Aug;49(3-4):118-23
Development of malaria vaccines that block transmission of parasites by
Hisaeda H, Yasutomo K.
Department of Immunology and Parasitology, The University of Tokushima School of
Malaria is still one of the infectious diseases urgently requiring control and
causes socioeconomic burdens on people residing in developing countries. Malaria
vaccines are expected to control the disease. However, there is no effective
vaccine available despite the intense efforts of malaria scientists. One
strategy for a malaria vaccine is to prevent parasite spread by means of
interfering with parasite development in mosquito vectors, which is the
so-called transmission-blocking vaccine (TBV). We will here review the current
progress of TBV.
J Am Mosq Control Assoc 2002 Sep;18(3):202-6
Low insecticide deposit rates detected during routine indoor residual spraying
for malaria vector control in two districts of Gokwe, Zimbabwe.
Masendu HT, Nziramasanga N, Muchechemera C.
Blair Research Institute. Ministry of Health and Child Welfare, Harare,
Questions have been raised about the quality of indoor residual spraying for
malaria vector control after the decentralization of the national malaria
control program in Zimbabwe. Given the critical role this control method plays,
we conducted an exercise to determine the amount of insecticide (mg active
ingredient/m2 of lambda-cyhalothrin) applied during routine house spraying.
Severe insecticide underdosing was detected. Spraying efficiency ranged between
63.4 and 76.1% on walls, and 52.7 and 63.2% on roofs. Differences between 2
districts suggested the problem originates from deficient training and lack of
pump calibration. Underdosing can undermine effective residual insecticide
activity and the expected reduction in disease transmission.
J Am Mosq Control Assoc 2002 Sep;18(3):178-85
Environmental factors associated with larval habitats of malaria vectors in
northern Kyunggi Province, Republic of Korea.
Claborn DM, Hshieh PB, Roberts DR, Klein TA, Zeichner BC, Andre RG.
Uniformed Services University of Health Sciences, Bethesda, MD 20814-4799, USA.
The larval habitats of malaria vectors near the Demilitarized Zone of the
Republic of Korea (ROK) were sampled from June through September 2000 to
determine larval abundance and to identify environmental factors associated with
high larval density. Six primary habitats were identified: rice fields,
irrigation ditches, drainage ditches, stream pools, irrigation pools, and
marshes. Most habitats harbored similar densities of larvae until August and
September, when population densities in rice fields declined and those in
irrigation pools increased. The primary vector in the ROK, Anopheles sinensis,
occurred in water with a wide range of values for environmental factors,
including pH, total dissolved solids, percent of surface covered with floating
vegetation, and nitrate and phosphate concentrations. No environmental factor or
combination of factors were found that were predictive of high larval densities.
This study suggests that larval Anopheles are capable of developing in a wide
range of stagnant, freshwater habitats in northern Kyunggi Province, ROK.
J Am Mosq Control Assoc 2002 Sep;18(3):138-51
Mosquito vector control and biology in Latin America--a twelfth symposium.
Clark GG, Martinez HQ.
Dengue Branch, Centers for Disease Control and Prevention, San Juan, PR
The 12th annual Latin American symposium presented by the American Mosquito
Control Association (AMCA) was held as part of the 68th Annual Meeting in
Denver, CO, in February 2002. The principal objective, as for the previous 11
symposia, was to promote participation in the AMCA by vector control
specialists, public health workers, and academicians from Latin America. This
publication includes summaries of 35 presentations that were given orally in
Spanish or presented as posters by participants from 7 countries in Latin
America. Topics addressed in the symposium included results from chemical and
biological control programs and studies; studies of insecticide resistance; and
population genetics, molecular, ecological, and behavioral studies of vectors of
dengue (Aedes aegypti and Aedes albopictus) and other arboviruses, malaria
(Anopheles albimanus and Anopheles pseudopunctipennis), leishmaniasis
(Lutzomyia), and Chagas Disease (Triatoma). Related topics included biology and
control of Culiseta inornata, Ochlerotatus taeniorhynchus, scorpions, Chironomus
plumosus, and Musca domestica.
Malar J 2002 Aug 14;1(1):10 [epub ahead of print]
Behavioural determinants of gene flow in malaria vector populations: Anopheles
gambiae males select large females as mates.
Okanda F, Dao A, Njiru B, Arija J, Akelo H, Toure Y, Odulaja A, Beier J, Githure
J, Yan G, Gouagna L, Knols B, Killeen G.
International Centre of Insect Physiology and Ecology, PO Box 30772, Nairobi,
Kenya. Email: [email protected]
BACKGROUND: Plasmodium-refractory mosquitoes are being rapidly developed for
malaria control but will only succeed if they can successfully compete for mates
when released into the wild. Pre-copulatory behavioural traits maintain genetic
population structure in wild mosquito populations and mating barriers have
foiled previous attempts to control malaria vectors through sterile male
release. METHODS: Varying numbers of virgin male and female Anopheles gambiae
Giles, from two strains of different innate sizes, were allowed to mate under
standardized conditions in laboratory cages, following which, the insemination
status, oviposition success and egg batch size of each female was assessed. The
influence of male and female numbers, strain combination and female size were
determined using logistic regression, correlation analysis and a simple
mechanistic model of male competition for females. RESULTS: Male An. gambiae
select females on the basis of size because of much greater fecundity among
large females. Even under conditions where large numbers of males must compete
for a smaller number of females, the largest females are more likely to become
inseminated, to successfully oviposit and to produce large egg batches.
CONCLUSIONS: Sexual selection, on the basis of size, could either promote or
limit the spread of malaria-refractory genes into wild populations and needs to
be considered in the continued development and eventual release of transgenic
vectors. Fundamental studies of behavioural ecology in malaria vectors such as
An. gambiae can have important implications for malaria control and should be
prioritised for more extensive investigation in the future.
Mol Ecol 2002 Oct;11(10):2183-2187
Genetic structure of Anopheles gambiae (Diptera: Culicidae) in Sao Tome
and Principe (West Africa): implications for malaria control.
Pinto J, Donnelly MJ, Sousa CA, Gil V, Ferreira C, Elissa N, Do Rosario VE,
Centro de Malaria e outras Doencas Tropicais, Instituto de Higiene e Medicina
Tropical, Universidade Nova de Lisboa. Rua da Junqueira 96 1349-008 Lisbon,
Portugal, Division of Parasite and Vector Biology, Liverpool School of Tropical
Medicine. Pembroke Place, Liverpool L3 5QA, UK, Division of Parasitic Diseases,
Centers for Disease Control and Prevention. MS; F22, 4770, Buford Highway,
Chamblee GA, USA, Unidade de Entomologia Medica, Instituto de Higiene e Medicina
Tropical, Universidade Nova de Lisboa. Rua da Junqueira 96 1349-008 Lisbon,
Portugal, Centro Nacional de Endemias, Ministerio de Saude. Caixa Postal 218 Sao
Tome, Democratic Republic of Sao Tome e Principe, Centre International de
Recherche Medicale de Franceville. BP 769 Franceville, Gabon.
The impact of a vector eradication programme, conducted in the 1980s, on
Anopheles gambiae populations from the islands of Sao Tome and Principe, was
evaluated by microsatellite DNA analysis. Significant genetic differentiation
was observed within and between the two islands and between the islands and a
population from Gabon, suggesting a degree of isolation between them. Large
estimates of long-term Ne suggested that the control programme did not affect
the effective population size of the vector. Heterozygosity tests were also not
consistent with a recent bottleneck.
Natl Med J India 2002 Jul-Aug;15(4):199-201
Passive malaria surveillance in a low endemic area of India: validation of a
clinical case definition.
Anand K, Kant S, Samantaray JC, Kapoor SK.
All India Institute of Medical Sciences, Ansari Nagar, New Delhi.
BACKGROUND: In India, 2.55 million cases of malaria were reported during 1997;
roughly one-third were due to Plasmodium falciparum. Malaria cases are
identified by passive and active surveillance and all patients with fever are
treated with chloroquine (10 mg/kg body weight). Since all fevers are not
malaria, this results in overtreatment and has a bearing in terms of the
parasites developing resistance. We aimed to test the validity of a clinical
algorithm for passive malaria surveillance by primary care doctors (fever with
pallor or splenomegaly) in a low endemic, Plasmodium vivax-predominant area of
Ballabgarh block in Faridabad District, Haryana. METHODS: Passive surveillance
was carried out at the general and paediatric outpatient departments (OPDs) of
Ballabgarh hospital. All persons with fever attending the OPD were examined for
the presence of fever, pallor and splenomegaly by the treating doctor. A blood
smear was prepared and examined in all these cases. RESULTS: A total of 3119
slides for malaria were made at Ballabgarh hospital but clinical details in the
requisition form were available for only 2616 patients who form the subjects of
this analysis. A total of 59 malaria cases (30 P. vivax cases and 29 P.
falciparum) were diagnosed. The presence of fever with pallor or splenomegaly
had a sensitivity of 28.8% (95% CI: 18.1-42.3); specificity of 88.6% (95% CI:
87.3-89.8), positive predictive value of 5.5% (95% CI: 3.3-8.8) and negative
predictive value of 98.2% (95% CI: 97.5-98.7). CONCLUSION: The algorithm did not
have sufficient sensitivity to detect malaria cases by passive surveillance.
Mol Biochem Parasitol 2002 Aug 28;123(2):85
Functional characterization of the acyl carrier protein (PfACP) and
beta-ketoacyl ACP synthase III (PfKASIII) from Plasmodium falciparum.
Waters N, Kopydlowski K, Guszczynski T, Wei L, Sellers P, Ferlan J, Lee P, Li Z,
Woodard C, Shallom S, Gardner M, Prigge S.
Division of Experimental Therapeutics, Walter Reed Army Institute of Research,
20910-5100, Silver Spring, MD, USA
The genome of the malaria parasite, Plasmodium falciparum, appears to contain
the proteins necessary for a Type II dissociated fatty acid biosynthetic system.
Here we report the functional characterization of two proteins from this system.
Purified recombinant acyl carrier protein (ACP) and beta-ketoacyl-ACP synthase
III (KASIII) from P. falciparum are soluble and active in a truncated form.
Malarial ACP is activated by the addition of a 4'-phosphopantetheine prosthetic
group derived from coenzyme A, generating holo-PfACP. Holo-PfACP is an effective
substrate for the transacylase activity of PfKASIII, but substitution of a key
active site cysteine in PfKASIII to alanine or serine abolishes enzymatic
activity. During the schizont stage of parasite development, there is a
significant up-regulation of the mRNAs corresponding to these proteins,
indicating an important metabolic requirement for fatty acids during this stage.
Immunol Lett 2002 Nov 1;84(2):133
Novel Plasmodium falciparum malaria vaccines: evidence-based searching
for variant surface antigens as candidates for vaccination against
Staalsoe T, Jensen A, Theander T, Hviid L.
Centre for Medical Parasitology, Department of Infectious Diseases, Department
of Clinical Microbiology, Copenhagen University Hospital, M7641 Rigshospitalet,
Institute for Medical Microbiology and Immunology, University of Copenhagen,
Blegdamsvej 9, 2100, Copenhagen, Denmark
Malaria vaccine development has traditionally concentrated on careful molecular,
biochemical, and immunological characterisation of candidate antigens. In
contrast, evidence of the importance of identified antigens in immunity to human
infection and disease has generally been limited to statistically significant
co-variation with protection rather than on demonstration of causal
relationships. We have studied the relationship between variant surface
antigen-specific antibodies and clinical protection from Plasmodium falciparum
malaria in general, and from pregnancy-associated malaria (PAM) in particular,
to provide robust evidence of a causal link between the two in order to allow
efficient and evidence-based identification of candidate antigens for malaria
Mol Cells 2002 Aug 31;14(1):158-62
Intragenomic length variation of the ribosomal DNA intergenic spacer in a
malaria vector, Anopheles sinensis.
Whang IJ, Jung J, Park JK, Min GS, Kim W.
School of Biological Sciences, Seoul National University, Korea.
We determined the complete sequences of six size variants of intergenic spacer
(IGS) region from one individual of the malaria vector mosquito species,
Anopheles sinensis. All six size variants observed in this study show almost the
same basic primary structure in which three repeat regions (A, B, and C) are
interspersed by highly conserved nonrepeating sections. In contrast to the
well-ordered subrepeating patterns found in A and C, the repeat region B
displays extremely variable and complicated profiles in the number and
arrangement of subrepeat units among different size classes. It is apparent that
the prominent level of length difference in the repeat regions B and C is
responsible for the intragenomic length variations of the IGS molecule observed
in the present study. High level of sequence homology and regularly arranged
repeating pattern of 11 to 14 bp motif sequences harbored within the B repeat
region allow us to consider that these motif sequences may be associated with
their potential role as a recombination site. Compared to those previously
published in other mosquito species, the IGS of A. sinensis showed a very unique
structural format in subrepeat patterns of the IGS region. This result suggests
that the structure and sequence profiles of the IGS region would provide useful
information for the exploitation of a convenient molecular marker to identify
morphologically complicated species complex and to characterize the genetic
variation of population. This suggestion is far from being conclusive at
present, but a further genetic study will bring more compelling evidences for
this pending issue.
Med Vet Entomol 2002 Sep;16(3):329-34
Presence of Anopheles culicifacies B in Cambodia established by the PCR-RFLP
assay developed for the identification of Anopheles minimus species A and C
and four related species.
Van Bortel W, Sochanta T, Harbach RE, Socheat D, Roelants P, Backeljau T,
Department of Parasitology, Prince Leopold Institute of Tropical Medicine,
Antwerpen, Belgium. Email: [email protected]
A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)
assay developed for identification of five species of the Anopheles minimus
Theobald group and a related mosquito species of the Myzomyia Series (Diptera:
Culicidae) was applied to morphologically identified adult female specimens
collected in Ratanakiri Province, north-eastern Cambodia. In addition to finding
An. aconitus Donitz, An. minimus species A and An. pampanai Buttiker & Beales,
some specimens showed a new restriction banding pattern. Siblings of specimens
that exhibited this new PCR-RFLP pattern were morphologically identified as An.
culicifacies James sensu lato. Based on nucleotide sequences of the ribonuclear
DNA internal transcribed spacer 2 region (ITS2) and the mitochondrial cytochrome
oxidase I gene (COI), these specimens were recognized as An. culicifacies
species B (sensu Green & Miles, 1980), the first confirmed record of the An.
culicifacies complex from Cambodia. This study shows that the PCR-RFLP assay can
detect species not included in the initial set-up and is capable of identifying
at least seven species of the Myzomyia Series, allowing better definition of
those malaria vector and non-vector anophelines in South-east Asia.
Med Vet Entomol 2002 Sep;16(3):321-7
Extensive multiple test centre evaluation of the VecTest malaria antigen
Ryan JR, Dave K, Collins KM, Hochberg L, Sattabongkot J, Coleman RE, Dunton RF,
Bangs MJ, Mbogo CM, Cooper RD, Schoeler GB, Rubio-Palis Y, Magris M, Romer LI,
Padilla N, Quakyi IA, Bigoga J, Leke RG, Akinpelu O, Evans B, Walsey M,
Patterson P, Wirtz RA, Chan AS.
Department of Entomology, Walter Reed Army Institute of Research, Silver Spring,
MD 20910-7500, USA. Email: [email protected]
To determine which species and populations of Anopheles transmit malaria in any
given situation, immunological assays for malaria sporozoite antigen can replace
traditional microscopical examination of freshly dissected Anopheles. We
developed a wicking assay for use with mosquitoes that identifies the presence
or absence of specific peptide epitopes of circumsporozoite (CS) protein of
Plasmodium falciparum and two strains of Plasmodium vivax (variants 210 and
247). The resulting assay (VecTest Malaria) is a rapid, one-step procedure using
a 'dipstick' test strip capable of detecting and distinguishing between P.
falciparum and P. vivax infections in mosquitoes. The objective of the present
study was to test the efficacy, sensitivity, stability and field-user
acceptability of this wicking dipstick assay. In collaboration with 16 test
centres world-wide, we evaluated more than 40 000 units of this assay, comparing
it to the standard CS ELISA. The 'VecTest Malaria' was found to show 92%
sensitivity and 98.1% specificity, with 97.8% accuracy overall. In accelerated
storage tests, the dipsticks remained stable for > 15 weeks in dry conditions up
to 45 degrees C and in humid conditions up to 37 degrees C. Evidently, this
quick and easy dipstick test performs at an acceptable level of reliability and
offers practical advantages for field workers needing to make rapid surveys of
Med Vet Entomol 2002 Sep;16(3):316-20
Effect of adult nutrition on the melanization immune response of the malaria
vector Anopheles stephensi.
Koella JC, Sorense FL.
Laboratoire de Parasitologie Evolutive, UMR 7103, Universite Pierre & Marie
Curie, Paris, France. Email: [email protected]
Two dietary resources - blood and sugar - were assessed for effects on the
melanization immune response of the mosquito Anopheles stephensi Liston
(Diptera: Culicidae) towards inoculated Sephadex beads (negatively charged
C-25). This melanization is conferred by genetic factors capable of making the
mosquito refractory to malaria parasites. If An. stephensi females had obtained
a bloodmeal one day before inoculation with a bead, the efficacy of their immune
response increased with the concentration of sugar ingested. At the highest
sugar concentration (6%) tested, 38% of the mosquitoes completely melanized
their bead, whereas at the lowest sugar concentration (2%), none of the
mosquitoes were able to melanize their bead completely. Among mosquitoes not
having a bloodmeal, the immuno-competence was low (c. 9% of the mosquitoes
completely melanized their bead) and independent of sugar concentration. The
observed interaction between these two resources indicates that both resources
are required for the Anopheles female to develop an effective melanization
Med Vet Entomol 2002 Sep;16(3):253-65
Molecular and morphological studies on the Anopheles minimus group of
mosquitoes in southern China: taxonomic review, distribution and malaria
Chen B, Harbach RE, Butlin RK.
School of Biology, The University of Leeds, UK. Email: [email protected]
Mosquitoes of the Anopheles minimus group (Diptera: Culicidae) from nine
Provinces of southern China were identified morphologically and by molecular
characterization, using single-strand conformation polymorphisms (SSCPs) and
sequence data for the D3 region of the 28S ribosomal DNA and the mitochondrial
COII locus. Species A and C (sensu Green et al., 1990) of the An. minimus
complex were found to be sympatric in Yunnan Province. Species A occurs eastward
from Yunnan through southern Guangxi, Hainan, Guangdong and Taiwan Provinces,
whereas species C occurs northward to northern Guangxi, Guizhou and Sichuan
Provinces. Morphological and molecular evidence (based on specimens from the
field and four isofemale lines) shows that An. minimus forms A and B (sensu Yu &
Li, 1984) are morphological variants of species A, which is accepted as An.
minimus Theobald sensu stricto (type-locality: Pokfulam, Hong Kong). The
so-called subspecies x of An. minimus (sensu Baba, 1950) is reinterpreted as An.
aconitus Donitz. The distribution and vector status of members of the An.
minimus group are discussed in relation to the historical and current
transmission of malaria and filariasis in China. Both An. minimus A and C have
been implicated as widespread vectors of malaria, whereas only species A has
been found in Hainan, where An. minimus s.l. was a vector of Bancroftian
filariasis. The presence of An. aconitus in Hainan and Yunnan Provinces is
confirmed, but the occurrence of An. varuna Iyengar and An. fluviatilis James,
which were previously recorded in China, could not be verified.
Lancet 2002 Aug 24;360(9333):610
Immunity to malaria after administration of ultra-low doses of red cells
infected with Plasmodium falciparum.
Pombo D, Lawrence G, Hirunpetcharat C, Rzepczyk C, Bryden M, Cloonan N, Anderson K, Mahakunkijcharoen Y, Martin L, Wilson D, Elliott S, Elliott S, Eisen D,
Weinberg J, Saul A, Good M.
Queensland Institute of Medical Research, Australian Centre for International
and Tropical Health and Nutrition, and Cooperative Research Centre for Vaccine
Technology, PO Royal Brisbane Hospital, Australia
Background The ability of T cells, acting independently of antibodies, to
control malaria parasite growth in people has not been defined. If such was
shown to be effective, an additional vaccine strategy could be pursued. Our aim
was to ascertain whether or not development of cell-mediated immunity to
Plasmodium falciparum blood-stage infection could be induced in human beings by
exposure to malaria parasites in very low density.Methods We enrolled five
volunteers from the staff at our research institute who had never had malaria.
We used a cryopreserved inoculum of red cells infected with P falciparum strain
3D7 to give them repeated subclinical infections of malaria that we then cured
early with drugs, to induce cell-mediated immune responses. We tested for
development of immunity by measurement of parasite concentrations in the blood
of volunteers by PCR of the multicopy gene STEVOR and by following up the
volunteers clinically, and by measuring antibody and cellular immune responses
to the parasite.Findings After challenge and a extended period without drug
cure, volunteers were protected against malaria as indicated by absence of
parasites or parasite DNA in the blood, and absence of clinical symptoms.
Immunity was characterised by absence of detectable antibodies that bind the
parasite or infected red cells, but by the presence of a proliferative T-cell
response, involving CD4+ and CD8+ T cells, a cytokine response, consisting of
interferon gamma but not interleukin 4 or interleukin 10, induction of high
concentrations of nitric oxide synthase activity in peripheral blood mononuclear
cells, and a drop in the number of peripheral natural killer T
cells.Interpretation People can be protected against the erythrocytic stage of
malaria by a strong cell-mediated immune response, in the absence of detectable
parasite-specific antibodies, suggesting an additional strategy for development
of a malaria vaccine
Arch Med Res 2002 Jul-Aug;33(4):416
The future outlook of antimalarial drugs and recent work on the treatment
Wilairatana P, Krudsood S, Treeprasertsuk S, Chalermrut K, Looareesuwan S.
Hospital for Tropical Diseases, Faculty of Tropical Medicine, Mahidol
University, Bangkok, Thailand
With the emergence of multidrug-resistant falciparum malaria, new drugs and
drugs in combination are urgently needed.New antimalarial drugs investigated at
the Hospital for Tropical Diseases of the Faculty of Tropical Medicine at
Mahidol University in Bangkok, Thailand in recent years for treatment of
uncomplicated and severe falciparum malaria are as follows: atovaquone, and
artemisinin derivatives (artesunate, artemether, arteether, and
dihydroartemisinin) combined with other antimalarials.Malarone((R)), artemisinin
derivatives combined with lumefantrine or doxycycline, and mefloquine combined
with tetracycline or doxycycline have been evaluated with improvement of the
cure rate in uncomplicated malaria. Artemisinin derivatives intravenously or
intrarectally combined with mefloquine may be alternatives to intravenous
quinine for treatment of severe malaria.In Thailand, drug treatment for
uncomplicated malaria consists of the combinations or artesunate plus mefloquine
or artemether plus lumefantrine or quinine plus tetracycline. In treatment of
severe malaria, antimalarial drugs of choice are intravenous quinine or
J Infect Dis 2002 Oct 1;186(7):1007-12
Profound Bias in Interferon-gamma and Interleukin-6 Allele Frequencies in
Western Kenya, Where Severe Malarial Anemia Is Common in Children.
Gourley IS, Kurtis JD, Kamoun M, Amon JJ, Duffy PE.
Department of Pathology and Laboratory Medicine, Hospital of the University of
Pennsylvania, Philadelphia, Pennsylvania, USA.
The intensity of malaria transmission is related to the pattern of malarial
disease observed in different regions, but populations may also differ in their
underlying predispositions to severe malarial anemia or cerebral malaria. In
western Kenya, where severe malarial anemia is much more common than cerebral
malaria, the distributions of tumor necrosis factor (TNF)-alpha, interleukin
(IL)-10, transforming growth factor (TGF)-beta, IL-6, and interferon (IFN)-gamma
alleles were examined in a cohort of young men. The cohort displayed a marked
bias toward genotypes associated with low expression of IFN-gamma and IL-6,
cytokines that, at high levels, have been implicated in malarial anemia and poor
malaria outcomes. By contrast, the frequency of the TNF-alpha -238A allele,
which has been associated with severe malarial anemia, was found to be similar
to the frequency previously reported in comparison populations in Africa and
elsewhere. IFN-gamma and IL-6 genotypes may play roles in the development of
severe malaria and could contribute to the relative frequency of severe malarial
anemia or cerebral malaria in exposed populations.
J Infect Dis 2002 Oct 1;186(7):999-1006
Origin and Dissemination of Plasmodium falciparum Drug-Resistance Mutations
in South America.
Cortese JF, Caraballo A, Contreras CE, Plowe CV.
Malaria Section, Center for Vaccine Development, University of Maryland School
of Medicine, Baltimore, Maryland, USA.
Multidrug resistance is a major obstacle to the control of Plasmodium falciparum
malaria, and its origins and modes of dissemination are imperfectly understood.
In this study, haplotyping and microsatellite analysis of malaria from 5 regions
of the South American Amazon support the conclusion that the parasite mutations
conferring mid- and high-level resistance to the antifolate combination
sulfadoxine-pyrimethamine have a common origin. Parasites harboring these
mutations are also found to share drug-resistance alleles that confer a unique
chloroquine resistance phenotype and to be similar at loci not linked to drug
resistance, although not genetically identical. Since the 1980s,
multidrug-resistant P. falciparum has spread in a north-northwest manner across
the continent, from an origin likely in the lower Amazon. This study highlights
the importance of continent-wide malaria-control policies and suggests that the
containment of resistance to the next generation of therapies may be feasible.